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Immunofluorescence Staining Using IBA1 and TMEM119 for Microglial Density, Morphology and Peripheral Myeloid Cell Infiltration Analysis in Mouse Brain
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Immunologie und Infektion
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JoVE Journal Immunologie und Infektion
Immunofluorescence Staining Using IBA1 and TMEM119 for Microglial Density, Morphology and Peripheral Myeloid Cell Infiltration Analysis in Mouse Brain

Immunofluorescence Staining Using IBA1 and TMEM119 for Microglial Density, Morphology and Peripheral Myeloid Cell Infiltration Analysis in Mouse Brain

29,283 Views

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10:40 min

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October 27, 2019

DOI:

10.3791/60510-v

DOI:
10.3791/60510-v

•

10:40 min
October 27, 2019

•

119 Views
, , , , ,
1Axe Neurosciences,Centre de Recherche du CHU de Québec-Université Laval, 2Département de Médecine Moléculaire, Faculté de Médecine,Université Laval, 3Integrated Program in Neuroscience,McGill University, 4Center for Brain Immunology and Glia (BIG),University of Virginia

Summary

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This protocol describes a step-by-step workflow for immunofluorescent costaining of IBA1 and TMEM119, in addition to analysis of microglial density, distribution, and morphology, as well as peripheral myeloid cell infiltration in mouse brain tissue.

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Cite this Article

González Ibanez, F., Picard, K., Bordeleau, M., Sharma, K., Bisht, K., Tremblay, M. Immunofluorescence Staining Using IBA1 and TMEM119 for Microglial Density, Morphology and Peripheral Myeloid Cell Infiltration Analysis in Mouse Brain. J. Vis. Exp. (152), e60510, doi:10.3791/60510 (2019).

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