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インビトロにおける胆道カネラ形成における細胞骨格の役割を研究する原発マウス肝細胞の単離と3Dコラーゲンサンドイッチ培養

JoVE Journal
Biologie

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JoVE Journal Biologie

Isolation and 3D Collagen Sandwich Culture of Primary Mouse Hepatocytes to Study the Role of Cytoskeleton in Bile Canalicular Formation In Vitro

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インビトロにおける胆道カネラ形成における細胞骨格の役割を研究する原発マウス肝細胞の単離と3Dコラーゲンサンドイッチ培養

10,684 Views

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10:12 min

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December 20, 2019

DOI:

10.3791/60507-v

DOI:

10.3791/60507-v

•

10:12 min

December 20, 2019

•

9 Views

Katerina Korelova, Marketa Jirouskova, Lenka Sarnova, Martin Gregor

¹Laboratory of Integrative Biology,Institute of Molecular Genetics of the Czech Academy of Sciences

Tags

3D Collagen Sandwich Culture Primary Mouse Hepatocytes Bile Canalicular Formation Cytoskeleton Liver Isolation Hepatocyte Isolation In Vitro Studies Perfusion Heparin DMEM Collagen I PBS Cell Culture

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