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Ultrasound-Guided Orthotopic Implantation of Murine Pancreatic Ductal Adenocarcinoma
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1Department of Medicine, Perelman School of Medicine,University of Pennsylvania, 2Pancreatic Cancer Mouse Hospital, Perelman School of Medicine,University of Pennsylvania, 3Abramson Cancer Center, Perelman School of Medicine,University of Pennsylvania, 4Parker Institute for Cancer Immunotherapy, Perelman School of Medicine,University of Pennsylvania
Kapitel
- 00:04Titel
- 01:08Pre-Surgical Preparation
- 02:17Ultrasound-Guided Pancreatic Ductal Adenocarcinoma (PDA) Cell Implantation
- 04:28Results: Representative Ultrasound-Guided Orthotopic Murine Pancreatic Ductal Adenocarcinoma Implantation
- 05:50Conclusion
We describe a protocol for the ultrasound guided implantation of murine-derived pancreatic ductal adenocarcinoma cell lines directly into the native tumor site. This approach resulted in pancreatic tumors detectable by ultrasound scanning within 2-4 weeks of injection, and significantly reduced the proportion tumor cell seeding on the peritoneal wall as compared to surgical orthotopic implantation.
