A protocol for 3D visualization of microscopic tissue structures by using an X-ray specific staining method designed for X-ray computed tomography is presented.
Busse, M., Müller, M., Kimm, M. A., Ferstl, S., Allner, S., Achterhold, K., Herzen, J., Pfeiffer, F. 3D Imaging of Soft-Tissue Samples using an X-ray Specific Staining Method and Nanoscopic Computed Tomography. J. Vis. Exp. (152), e60251, doi:10.3791/60251 (2019).