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Visualizing Surface T-Cell Receptor Dynamics Four-Dimensionally Using Lattice Light-Sheet Microscopy
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JoVE Journal Biochemie
Visualizing Surface T-Cell Receptor Dynamics Four-Dimensionally Using Lattice Light-Sheet Microscopy

Visualizing Surface T-Cell Receptor Dynamics Four-Dimensionally Using Lattice Light-Sheet Microscopy

7,745 Views

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09:24 min

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January 30, 2020

DOI:

10.3791/59914-v

DOI:
10.3791/59914-v

•

09:24 min
January 30, 2020

•

1 Views
,
1Committee on Cancer Biology,The University of Chicago, 2Pritzker School of Molecular Engineering,The University of Chicago

Summary

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The goal of this protocol is to show how to use Lattice Light-Sheet Microscopy to four-dimensionally visualize surface receptor dynamics in live cells. Here T cell receptors on CD4+ primary T cells are shown.

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Four-dimensional ImagingLattice Light-sheet MicroscopyLive Cell ImagingReal-time TrackingMolecular DynamicsT-cell ReceptorAntigen-presenting CellsSample PreparationCell Labeling

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Cite this Article

Rosenberg, J., Huang, J. Visualizing Surface T-Cell Receptor Dynamics Four-Dimensionally Using Lattice Light-Sheet Microscopy. J. Vis. Exp. (155), e59914, doi:10.3791/59914 (2020).

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