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Purification of High Yield Extracellular Vesicle Preparations Away from Virus
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Immunologie und Infektion
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JoVE Journal Immunologie und Infektion
Purification of High Yield Extracellular Vesicle Preparations Away from Virus

Purification of High Yield Extracellular Vesicle Preparations Away from Virus

DOI:
10.3791/59876-v

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00:07 min

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September 12, 2019

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, , , , , , , ,
1Laboratory of Molecular Virology, School of Systems Biology,George Mason University, 2American Type Culture Collection (ATCC), 3ATCC Cell Systems, 4Ceres Nanosciences, Inc, 5Department of Immunology and Nano-medicine, Herbert Wertheim College of Medicine,Florida International University

Kapitel

  • 00:04Titel
  • 01:00Filtration and Precipitation of Extracellular Vesicles
  • 02:25Construction of a Density Gradient and Enrichment of EV Fractions
  • 04:06Preparation of Nanoparticle Pellet for Downstream Assay
  • 05:05Results: Increased EV Yield and Characterization of EVs
  • 06:09Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

This protocol isolates extracellular vesicles (EVs) away from virions with high efficiency and yield by incorporating EV precipitation, density gradient ultracentrifugation, and particle capture to allow for a streamlined workflow and a reduction of starting volume requirements, resulting in reproducible preparations for use in all EV research.

Tags

Extracellular VesiclesEVsVirus-free EV PreparationsEV PurificationUltracentrifugationPEG PrecipitationIodixanol Density GradientNanoparticle Enrichment

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