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Entwicklungsbiologie
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在无进料和异种定义的条件下,从人类多能干细胞中血性内皮物分化
JoVE Journal
Entwicklungsbiologie
This content is Free Access.
JoVE Journal
Entwicklungsbiologie
Hemogenic Endothelium Differentiation from Human Pluripotent Stem Cells in A Feeder- and Xeno-free Defined Condition
Please note that all translations are automatically generated.
Click here for the English version.
在无进料和异种定义的条件下,从人类多能干细胞中血性内皮物分化
DOI:
10.3791/59823-v
•
09:00 min
•
June 16, 2019
•
Ryo Ohta*
1
,
Ryohichi Sugimura*
1
,
Akira Niwa
,
Megumu K. Saito
1
Center for iPS cell research and application (CiRA)
Kapitel
00:04
Titel
01:11
Pluripotent Stem Cell Colony Formation
02:45
Hemogenic Induction and Isolation of Hemogenic Endothelium
04:08
Induction of Endothelial-to-hematopoietic Transition (EHT)
05:00
Flow Cytometry Analysis of Hematopoietic Cells
06:27
Colony Forming Unit Assay of Hematopoietic Cells
07:30
Results: Hemogenic Induction
08:19
Conclusion
Summary
Automatische Übersetzung
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Automatische Übersetzung
在这里,我们提出了一个协议,以精确形成血源内皮,从人类多能干细胞在馈送和异种条件下。该方法在治疗性化合物的疾病建模和筛选中有着广泛的应用。
Tags
Hemogenic Endothelium
Pluripotent Stem Cells
Feeder-free
Xeno-free
Blood Cells
Hematology
Immunology
Genetic Intervention
Gene Editing
LM511-E8
PSC Colonies
Dissociation Buffer
Differentiation
Hypoxia
Culture Conditions
Article
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