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Real-time i Vitro overvågning af lugtstof Receptor aktivering af en lugtstof i fasen for damp
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1Department of Molecular Genetics and Microbiology,Duke University Medical Center, 2Department of Biotechnology and Life Science,Tokyo University of Agriculture and Technology, 3Department of Neurobiology,Duke University Medical Center, 4Department of Mechanical Systems, Engineering,Tokyo University of Agriculture and Technology, 5Institute of Global Innovation Research,Tokyo University of Agriculture and Technology, 6Duke Institute for Brain Sciences,Duke University
Kapitel
- 00:04Titel
- 00:42Hana3A Cells Culture
- 02:26Preparation of the Cells for Transfection
- 03:23Plasmid Transfection
- 05:01Substrate Incubation
- 06:07Odorant Stimulation and Data Analysis
- 08:07Results: Real-time Activation and Dose Dependent Responses
- 09:21Conclusion
Fysiologisk, aktiveres lugtstof receptorer af lugtstof molekyler inhaleret i fasen for damp. Dog anvender mest in vitro-systemer flydende fase lugtstof stimulation. Vi præsenterer her, en metode, der giver mulighed for real-time in vitro-overvågning af lugtstof receptor aktivering efter lugtstof stimulation i vapor fase.
