/
/
Realtid Live-cell flödescytometri att undersöka kalcium tillströmning, Pore bildandet och fagocytos av P2X7-receptorer i vuxen neurala stamceller
This content is Free Access.
1Griffith Institute for Drug Discovery,Griffith University, 2Australian Institute for Bioengineering and Nanotechnology,University of Queensland, 3Discipline of Anatomy and Histology, School of Medical Science,University of Sydney, 4Bosch Institute,University of Sydney, 5Applied Neurosciences Program, Peter Duncan Neurosciences Research Unit,St. Vincent’s Centre for Applied Medical Research, 6School of Medical Sciences,The University of New South Wales (UNSW) Medicine, Sydney, New South Wales, 7School of Environment and Science,Griffith University, Brisbane, Queensland, 8Florey Institute of Neuroscience and Mental Health,University of Melbourne
Kapitel
- 00:04Titel
- 00:58Preparation of a Single-cell Suspension of Neural Progenitor Cells for Analysis by Flow Cytometry
- 02:11Measuring Calcium Influx by Live-cell Flow Cytometry
- 05:21Measuring Pore Formation by Live-cell Flow Cytometry
- 07:02Measuring Phagocytosis by Live-cell Flow Cytometry
- 09:02Results: Analyses of Calcium Influx, Pore Formation, and Phagocytosis by Live-cell Flow Cytometry
- 10:46Conclusion
Att ge encelliga känslighet, är realtid flödescytometri unikt lämpade att kvantifiera multimodala receptor funktioner levande kulturer. P2X7 receptor funktionen använder vuxen neurala stamceller, och bedömdes via kalcium tillströmning upptäcks av kalcium indikator dye, transmembrana pore bildandet av etidiumbromid bromid upptag och fagocytos med fluorescerande latex pärlor.
