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Realtids Live-celle flowcytometri at undersøge Calcium tilstrømning, Pore dannelse og fagocytose af P2X7 receptorer i voksne neurale stamceller
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1Griffith Institute for Drug Discovery,Griffith University, 2Australian Institute for Bioengineering and Nanotechnology,University of Queensland, 3Discipline of Anatomy and Histology, School of Medical Science,University of Sydney, 4Bosch Institute,University of Sydney, 5Applied Neurosciences Program, Peter Duncan Neurosciences Research Unit,St. Vincent’s Centre for Applied Medical Research, 6School of Medical Sciences,The University of New South Wales (UNSW) Medicine, Sydney, New South Wales, 7School of Environment and Science,Griffith University, Brisbane, Queensland, 8Florey Institute of Neuroscience and Mental Health,University of Melbourne
Kapitel
- 00:04Titel
- 00:58Preparation of a Single-cell Suspension of Neural Progenitor Cells for Analysis by Flow Cytometry
- 02:11Measuring Calcium Influx by Live-cell Flow Cytometry
- 05:21Measuring Pore Formation by Live-cell Flow Cytometry
- 07:02Measuring Phagocytosis by Live-cell Flow Cytometry
- 09:02Results: Analyses of Calcium Influx, Pore Formation, and Phagocytosis by Live-cell Flow Cytometry
- 10:46Conclusion
Giver én celle følsomhed, er real-time flowcytometri unikt tilpasset til at kvantificere multimodale receptor funktioner af levende kulturer. Ved hjælp af voksne neurale stamceller, blev P2X7 receptor funktionen vurderet via calcium tilstrømning registreres af calcium indikator farvestof, transmembrane pore dannelsen af ethidium bromid optagelse og fagocytose ved hjælp af fluorescerende latex perler.
