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Immunologie und Infektion
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Generation, Amplification, and Titration of Recombinant Respiratory Syncytial Viruses
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Immunologie und Infektion
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JoVE Journal Immunologie und Infektion
Generation, Amplification, and Titration of Recombinant Respiratory Syncytial Viruses

Generation, Amplification, and Titration of Recombinant Respiratory Syncytial Viruses

DOI:
10.3791/59218-v

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11:48 min

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April 04, 2019

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, , , , , , ,
1UMR 1173 Institut national de la santé et de la recherche médicale (INSERM),Université de Versailles St. Quentin, 2UR892 Institut national de la recherche agronomique (INRA), Unité de virologie et immunologie moléculaires, 3Assistance Publique–Hôpitaux de Paris (AP-HP), Hôpital Ambroise Paré, Laboratoire de Microbiologie

Kapitel

  • 00:04Titel
  • 00:40Rescue and First Passage of Recombinant Virus
  • 04:17Amplification of the Rescued Viruses
  • 06:30Plaque Titration Assay
  • 09:41Results: An Optimized Titration Assay of Human RSV
  • 11:22Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

We describe a method for generating and amplifying genetically modified respiratory syncytial viruses (RSVs) and an optimized plaque assay for RSVs. We illustrate this protocol by creating two recombinant viruses that respectively allow quantification of RSV replication and live analysis of RSV inclusion bodies and inclusion bodies-associated granules dynamics.

Tags

Recombinant Respiratory Syncytial VirusVirus RescueVirus AmplificationVirus TitrationBSR-T7/5 CellsTransfectionHEp-2 CellsVirus Stock GenerationVirological Studies

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