Journal
/
Entwicklungsbiologie
/
Isolation of Embryonic Tissues and Formation of Quail-Chicken Chimeric Organs Using The Thymus Example
JoVE Journal
Entwicklungsbiologie
Author Produced
Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich.  Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
JoVE Journal Entwicklungsbiologie
Isolation of Embryonic Tissues and Formation of Quail-Chicken Chimeric Organs Using The Thymus Example

Isolation of Embryonic Tissues and Formation of Quail-Chicken Chimeric Organs Using The Thymus Example

DOI:
10.3791/58965-v

•

14:23 min

•

February 16, 2019

•

,
1Instituto de Histologia e Biologia do Desenvolvimento, Faculdade de Medicina,Universidade de Lisboa

Kapitel

  • 00:00Titel
  • 02:22Isolation of Quail Endoderm Containing the Prospective Domain of the Thymic Rudiment
  • 07:41Isolation of Chicken Somatopleura Mesoderm
  • 10:36In Vitro Organotypic Assay: Heterospecific Association of Quail ¾PP Endoderm and Chicken Somatopleura Mesoderm
  • 12:13Representative Results
  • 13:49Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

This article provides a method to isolate pure embryonic tissues from quail and chicken embryos that can be combined to form ex vivo chimeric organs.

Tags

Quail-chicken Chimeric OrgansThymus FormationIn Vitro Organotypic AssayTranscriptome AnalysisDevelopmental ProcessesPharyngeal PouchesSomatopleura Mesoderm

Article

Embed

ZUR WIEDERGABELISTE HINZUFÜGEN

Usage Statistics

Verwandte Videos

Transcriptome Profiling of In-Vivo Produced Bovine Pre-implantation Embryos Using Two-color Microarray Platform

Epigenetic Regulation of Cardiac Differentiation of Embryonic Stem Cells and Tissues

Isolation and Differentiation of Adipose-Derived Stem Cells from Porcine Subcutaneous Adipose Tissues

Isolation of Murine Embryonic Hemogenic Endothelial Cells

Culture of Murine Embryonic Metatarsals: A Physiological Model of Endochondral Ossification

Protocols for Visualizing Steroidogenic Organs and Their Interactive Organs with Immunostaining in the Fruit Fly Drosophila melanogaster

Using In Vivo and Tissue and Cell Explant Approaches to Study the Morphogenesis and Pathogenesis of the Embryonic and Perinatal Aorta

Two-step Approach to Explore Early- and Late-stages of Organ Formation in the Avian Model: The Thymus and Parathyroid Glands Organogenesis Paradigm

Three-dimensional Organotypic Cultures of Vestibular and Auditory Sensory Organs

A Familial Hypercholesterolemia Human Liver Chimeric Mouse Model Using Induced Pluripotent Stem Cell-derived Hepatocytes

Read Article