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cd8+ t 细胞诱导体外肿瘤细胞凋亡的实时检测--研究肿瘤浸润髓内细胞的免疫抑制作用
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Krebsforschung
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JoVE Journal Krebsforschung
Real Time Detection of In Vitro Tumor Cell Apoptosis Induced by CD8+ T Cells to Study Immune Suppressive Functions of Tumor-infiltrating Myeloid Cells
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cd8+ t 细胞诱导体外肿瘤细胞凋亡的实时检测--研究肿瘤浸润髓内细胞的免疫抑制作用

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09:57 min

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January 29, 2019

DOI:

10.3791/58841-v

DOI:
10.3791/58841-v

•

09:57 min
January 29, 2019

•

7 Views
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1Royal (Dick) School of Veterinary Studies and Roslin Institute,University of Edinburgh, 2MRC Centre for Reproductive Health,University of Edinburgh, 3Edinburgh Phenotypic Assay Centre,University of Edinburgh, 4Cancer Research UK Edinburgh Centre, MRC Institute of Genetics, Molecular Medicine,University of Edinburgh

Summary

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我们在这里描述了一个方案,研究细胞的预激活 cd8+ t 细胞对癌细胞通过实时显微镜检测凋亡癌细胞。该方案可以研究骨髓细胞诱导的 t 细胞抑制背后的机制, 并评估旨在通过阻断免疫抑制骨髓细胞来补充 t 细胞的化合物。

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Tumor Cell ApoptosisCD8+ T CellsTumor-infiltrating Myeloid CellsImmune SuppressionCancer ImmunologyAutoimmune DiseasesCell-to-cell InteractionsCheckpoint InhibitorCell CultureTarget CellsT-cell Activation

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Kitamura, T., Doughty-Shenton, D., Pollard, J. W., Carragher, N. O. Real Time Detection of In Vitro Tumor Cell Apoptosis Induced by CD8+ T Cells to Study Immune Suppressive Functions of Tumor-infiltrating Myeloid Cells. J. Vis. Exp. (143), e58841, doi:10.3791/58841 (2019).

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