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In Vivo Calcium Imaging of Lateral-line Hair Cells in Larval Zebrafish
JoVE Journal
Neurowissenschaften
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JoVE Journal Neurowissenschaften
In Vivo Calcium Imaging of Lateral-line Hair Cells in Larval Zebrafish

In Vivo Calcium Imaging of Lateral-line Hair Cells in Larval Zebrafish

DOI:
10.3791/58794-v

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08:51 min

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November 28, 2018

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,
1Section on Sensory Cell Development and Function,NIDCD/National Institutes of Health, 2National Institutes of Health-Johns Hopkins University Graduate Partnerships Program

Kapitel

  • 00:04Titel
  • 00:33Immobilizing Larva to Imaging Chamber
  • 02:06Injection of α-Bungarotoxin into the Heart Cavity to Paralyze Larva
  • 03:47Alignment of Larva and Fluid-jet
  • 07:03Single-plane Imaging Acquisition
  • 07:34Results: Spatiotemporal Visualization of Presyntaptic GCaMP6s Signals During Fluid-jet Stimulation
  • 08:22Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

The zebrafish is a model system that has many valuable features including optical clarity, rapid external development, and, of particular importance to the field of hearing and balance, externally located sensory hair cells. This article outlines how transgenic zebrafish can be used to assay both hair-cell mechanosensation and presynaptic function in toto.

Tags

Keywords: In VivoCalcium ImagingLateral-lineHair CellsLarvalZebrafishMechanotransductionPresynaptic ActivitySensory Hair CellsTungsten WireHead PinsTail PinsAlpha BungarotoxinHeart InjectionMicromanipulatorPressure Injector

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