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Tilapia 호수 바이러스를 사용 하 여 기존의 RT-PCR 및 SYBR의 탐지 RT 정량 그린
JoVE Journal
Umwelt
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JoVE Journal Umwelt
Detection of Tilapia Lake Virus Using Conventional RT-PCR and SYBR Green RT-qPCR
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Tilapia 호수 바이러스를 사용 하 여 기존의 RT-PCR 및 SYBR의 탐지 RT 정량 그린

25,995 Views

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10:55 min

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November 10, 2018

DOI:

10.3791/58596-v

DOI:
10.3791/58596-v

•

10:55 min
November 10, 2018

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11 Views
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1Institute of Veterinary Bacteriology Vetsuisse Faculty,University of Bern, 2Department of Veterinary Microbiology and Immunology and Center for Advanced Studies for Agriculture and Food, Kasetsart University Institute for Advanced Studies,Kasetsart University

Tags

Tilapia Lake VirusRT-PCRSYBR Green RT-qPCRSample CollectionRNA ExtractionReal-time PCRVirus DetectionTilapiaAquacultureEmerging Viral DiseaseFood Security

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Nicholson, P., Rawiwan, P., Surachetpong, W. Detection of Tilapia Lake Virus Using Conventional RT-PCR and SYBR Green RT-qPCR. J. Vis. Exp. (141), e58596, doi:10.3791/58596 (2018).

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