/

Entwicklungsbiologie

/

用扫描电镜和全贴免疫荧光技术可视化小鼠胚胎中的节点和外型板

JoVE Journal
Entwicklungsbiologie

This content is Free Access.

JoVE Journal Entwicklungsbiologie

Visualizing the Node and Notochordal Plate In Gastrulating Mouse Embryos Using Scanning Electron Microscopy and Whole Mount Immunofluorescence

Please note that all translations are automatically generated. Click here for the English version.

用扫描电镜和全贴免疫荧光技术可视化小鼠胚胎中的节点和外型板

DOI:

10.3791/58321-v

•

09:36 min

•

November 06, 2018

•

Cally Xiao^2,3, Frank Nitsche, Hisham Bazzi³

¹Graduate Program in Pharmacology and Experimental Therapeutics,University of Cologne, ²Department of Dermatology and Venereology,University Hospital of Cologne, ³Cologne Cluster of Excellence in Cellular Stress Responses in Aging-Associated Diseases (CECAD),University of Cologne, ⁴Department of General Ecology, Institute for Zoology, Biocenter Cologne,University of Cologne

Kapitel

00:04Titel
00:59Scanning Electron Microscopy of the Mouse Embryonic Node
04:17Whole Mount Immunofluorescence of the Mouse Node and Notochordal Plate
08:06Results: Strip1 Mutant Embryos Display Irregular Nodes and Abnormal F-Actin Organization in the Axial Mesoderm
08:46Conclusion

Summary

Automatische Übersetzung

节点和脊索板是小鼠胚胎发育过程中的瞬态信号组织者, 可以使用多种技术进行可视化。在这里, 我们详细地描述了如何执行两种技术来研究它们的结构和形态发生: 1) 扫描电子显微镜 (sem);和 2) 全安装免疫荧光 (wmif)。

Tags

Keywords: Node Notochordal Plate Mouse Embryo Scanning Electron Microscopy (SEM)Whole Mount Immunofluorescence Embryonic Development Dissection Fixation Dehydration Genotyping Reichert’s Membrane Yolk Sac

Article

Embed

ZUR WIEDERGABELISTE HINZUFÜGEN

Usage Statistics

Verwandte Videos

对比成像的小鼠胚胎使用高频超声

健康和受伤斑马鱼脑的大型扫描透射电子显微镜（Nanotomy）

多目标显色全安装<em>原位</em>在比较基因表达域杂交<em>果蝇</em>胚胎

方法来检查淋巴腺和血细胞中<em>果蝇</em>幼虫

器官培养和鼠标沃尔弗导管的全山免疫染色

全贴装荧光原位杂交和免疫荧光法在斑马鱼中 Multiciliated 细胞的可视化

小鼠胚胎心肌内祖群的定量全贴免疫荧光分析

用于在大容量中查找特定细胞目标的串行剖面的多模式分层成像

培养小鼠卵巢的全贴装免疫荧光和卵泡定量研究

全山荧光在小鼠胚胎发生过程中心脏室发育的分析

Read Article