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心脏外免疫细胞的分离与鉴定
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Immunologie und Infektion
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JoVE Journal Immunologie und Infektion
Isolation and Identification of Extravascular Immune Cells of the Heart
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心脏外免疫细胞的分离与鉴定

12,030 Views

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08:24 min

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August 23, 2018

DOI:

10.3791/58114-v

DOI:
10.3791/58114-v

•

08:24 min
August 23, 2018

•

21 Views
, ,
1Toronto General Hospital Research Institute,University Health Network (UHN), 2Dept of Laboratory Medicine and Pathobiology,University of Toronto, 3Dept of Immunology,University of Toronto, 4Peter Munk Cardiac Centre, 5Ted Rogers Centre for Heart Research

Summary

Automatically generated

该协议提出了一种简便、有效的方法, 用于分离、鉴定和量化在小鼠心肌中的免疫细胞在稳态或炎症状态下的存在。该协议结合酶和机械消化的生成单细胞悬浮, 可以进一步分析的流式细胞术。

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Cite this Article

Aronoff, L., Epelman, S., Clemente-Casares, X. Isolation and Identification of Extravascular Immune Cells of the Heart. J. Vis. Exp. (138), e58114, doi:10.3791/58114 (2018).

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