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Caenorhabditis Sieve: A Low-tech Instrument and Methodology for Sorting Small Multicellular Organisms
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Entwicklungsbiologie
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JoVE Journal Entwicklungsbiologie
Caenorhabditis Sieve: A Low-tech Instrument and Methodology for Sorting Small Multicellular Organisms

Caenorhabditis Sieve: A Low-tech Instrument and Methodology for Sorting Small Multicellular Organisms

DOI:
10.3791/58014-v

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04:39 min

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July 04, 2018

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, , , ,
1Department of Biology and Wildlife,University of Alaska Fairbanks, 2Institute of Arctic Biology,University of Alaska Fairbanks, 3Department of Chemistry and Biochemistry,University of Alaska Fairbanks, 4Departments of Biology and Chemistry,Earlham College, 5Department of Biological Sciences, College of Natural Science and Mathematics,California State University Long Beach

Kapitel

  • 00:04Titel
  • 00:55Caenorhabditis Sieve Construction
  • 01:58Caenorhabditis Sieve Use
  • 03:30Results: Caenorhabditis Sieve Does Not Impact Healthspan Metrics
  • 04:17Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

The current protocol includes a methodology for the sorting and cleaning of age-matched populations of Caenorhabditis elegans. It uses a simple, inexpensive, and efficient custom-made tool to obtain a large experimental population of nematodes for research.

Tags

C.elegansMolecular Biology AssaysDevelopmental StagesBody Size SelectionLow-tech InstrumentSortingMulticellular OrganismsNylon Monofilament MeshCyanoacrylate GlueM9 BufferAuger PlateCaenorhabditis Sieve

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