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Journal
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Genetik
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哺乳动物系统 CRISPR 指南 RNA 克隆
JoVE Journal
Genetik
This content is Free Access.
JoVE Journal
Genetik
CRISPR Guide RNA Cloning for Mammalian Systems
Please note that all translations are automatically generated.
Click here for the English version.
哺乳动物系统 CRISPR 指南 RNA 克隆
DOI:
10.3791/57998-v
•
06:48 min
•
October 02, 2018
•
Sathiji Nageshwaran*
1,2
,
Alejandro Chavez*
1,2,3
,
Nan Cher Yeo
2
,
Xiaoge Guo
2
,
Alissa Lance-Byrne
,
Angela Tung
,
James J. Collins
4,5,6,7
,
George M. Church
2
1
Wyss Institute for Biologically Inspired Engineering
,
Harvard University
,
2
Department of Genetics
,
Harvard Medical School
,
3
Department of Pathology
,
Massachusetts General Hospital
,
4
Institute for Medical Engineering & Science
,
Massachusetts Institute of Technology
,
5
Synthetic Biology Center
,
Massachusetts Institute of Technology
,
6
Department of Biological Engineering
,
Massachusetts Institute of Technology
,
7
Broad Institute
Kapitel
00:04
Titel
00:25
Preparation of the Primers and Expression Vectors
02:05
Ligating the Annealed gRNA Oligonucleotides
02:59
Transformation of
E. coli
04:03
PCR Analysis
05:35
Results: CRISPR Guided RNA Cloning
06:23
Conclusion
Summary
Automatische Übersetzung
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Automatische Übersetzung
这里概述了一种简单、高效、经济高效的 sgRNA 克隆方法。
Tags
CRISPR
Guide RNA
Cloning
Mammalian System
Plasmid
Single-guide RNA
Oligonucleotide
Ligation
E. Coli
Competent Cells
Transformation
Article
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