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基因型衰老过程中正常人成纤维细胞活性氧和衰老相关分泌表型的定量测定
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Entwicklungsbiologie
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JoVE Journal Entwicklungsbiologie
A Quantitative Measurement of Reactive Oxygen Species and Senescence-associated Secretory Phenotype in Normal Human Fibroblasts During Oncogene-induced Senescence
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基因型衰老过程中正常人成纤维细胞活性氧和衰老相关分泌表型的定量测定

7,951 Views

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13:59 min

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August 12, 2018

DOI:

10.3791/57890-v

DOI:
10.3791/57890-v

•

13:59 min
August 12, 2018

•

3 Views
, ,
1Department of Biochemistry, College of Medicine,Dong-A University, 2Peripheral Neuropathy Research Center, College of Medicine,Dong-A University

Summary

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胞内活性氧在细胞衰老诱导中起着重要作用。在这里, 我们描述了一个敏感的测定细胞衰老过程中的 ROS 水平。我们还提供了评估衰老相关分泌表型的协议, 据报告, 这有助于各种年龄相关的功能障碍。

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Kim, Y. Y., Um, J., Yun, J. A Quantitative Measurement of Reactive Oxygen Species and Senescence-associated Secretory Phenotype in Normal Human Fibroblasts During Oncogene-induced Senescence. J. Vis. Exp. (138), e57890, doi:10.3791/57890 (2018).

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