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Optogenetics Identification of a Neuronal Type with a Glass Optrode in Awake Mice
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JoVE Journal Neurowissenschaften
Optogenetics Identification of a Neuronal Type with a Glass Optrode in Awake Mice

Optogenetics Identification of a Neuronal Type with a Glass Optrode in Awake Mice

DOI:
10.3791/57781-v

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07:51 min

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June 28, 2018

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, , ,
1Department of Physiology,Kanazawa Medical University

Kapitel

  • 00:04Titel
  • 00:44Construction of the Glass Optrode Holder
  • 01:29Head Post Installation
  • 04:04Craniotomy
  • 05:01Electrophysiological Recording
  • 06:33Results: The Light-evoked Responses in the IC of Awake VGAT-ChR2 Mice
  • 07:29Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

This work introduces a method to perform an optogenetic single-unit recording reliably from an awake mouse using a custom-made glass optrode.

Tags

OptogeneticsNeurowissenschaftenNeuronal Type IdentificationGlass OptrodeIn VivoTransgenic MouseChannelrhodopsin-IIStereotaxic SurgeryDental CementHead Post

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