Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich. Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
全基因组对真核生物转录误差的监测
1Center for Mitochondrial and Epigenomic Medicine,Children’s Hospital of Philadelphia, 2Department of Cellular and Molecular Biology,University of Pennsylvania, 3Department of Biological Sciences,Mississippi State University, 4Center for Mechanisms of Evolution, Biodesign Institute,Arizona State University
Kapitel
- 00:04Titel
- 00:38RNA Circularization and Rolling Circle Reverse Transcription
- 02:51Second Strand cDNA Synthesis and Repair
- 03:45Adapter Ligation and Size Selection
- 06:21PCR Amplification and Final Bead Purification
- 07:53Results: Quality Control, Bio-Informatic Analysis, and Expected Results from the C-seq Assay
- 08:54Conclusion
