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Ablation of a Neuronal Population Using a Two-photon Laser and Its Assessment Using Calcium Imaging and Behavioral Recording in Zebrafish Larvae
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JoVE Journal Neurowissenschaften
Ablation of a Neuronal Population Using a Two-photon Laser and Its Assessment Using Calcium Imaging and Behavioral Recording in Zebrafish Larvae

Ablation of a Neuronal Population Using a Two-photon Laser and Its Assessment Using Calcium Imaging and Behavioral Recording in Zebrafish Larvae

DOI:

10:29 min

June 02, 2018

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Kapitel

  • 00:04Titel
  • 00:51Ablation of a Subpopulation of Neurons Using a Two-photon Laser Microscope
  • 04:47Calcium imaging to Record Prey-Evoked Neuronal Activity in the Pretectum-Ablated Zebrafish Larvae
  • 06:38Assessment of Behavioral Consequences Following Laser Ablation
  • 07:30Results: Assessment of Laser Ablated Pretectal Neurons with Calcium Imaging
  • 09:05Conclusion

Summary

Automatische Übersetzung

Here, we present a protocol to ablate a genetically labeled subpopulation of neurons by a two-photon laser from Zebrafish larvae.

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