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CARIP-Seq and ChIP-Seq: Methods to Identify Chromatin-Associated RNAs and Protein-DNA Interactions in Embryonic Stem Cells
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Entwicklungsbiologie
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JoVE Journal Entwicklungsbiologie
CARIP-Seq and ChIP-Seq: Methods to Identify Chromatin-Associated RNAs and Protein-DNA Interactions in Embryonic Stem Cells

CARIP-Seq and ChIP-Seq: Methods to Identify Chromatin-Associated RNAs and Protein-DNA Interactions in Embryonic Stem Cells

DOI:
10.3791/57481-v

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11:13 min

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May 25, 2018

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1Department of Oncology,Wayne State University School of Medicine, 2Karmanos Cancer Institute,Wayne State University School of Medicine

Kapitel

  • 00:04Titel
  • 01:02Crosslinking of Embryonic Stem Cells for ChIP and CARIP
  • 02:29Chromatin Sonication for ChIP and CARIP
  • 03:48ChIP and CARIP Process
  • 07:12Double-stranded cDNA Synthesis for CARIP-Seq
  • 08:53Use of ChIP-Seq and CARIP-Seq to Study Global Protein-DNA Interactions and Chromatin-Associated RNAs in Embryonic Stem Cells
  • 10:43Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

Here, we describe methods to perform ChIP-Seq and CARIP-Seq, including library preparation for next-generation sequencing, to generate global epigenomic and chromatin-associated RNA maps in ES cells.

Tags

CARIP-SeqChIP-SeqChromatin-associated RNAsProtein-DNA InteractionsEmbryonic Stem CellsEpigeneticsTranscription FactorsEpigenetic RegulatorsHistone ModificationsChromatin ImmunoprecipitationSonicationFormaldehyde Cross-linkingGlycinePhosphate Buffered SalineTrypsinizationMEF MediumFetal Bovine SerumSonication BufferSodium Dodecyl Sulfate

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