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Fetal Mouse Cardiovascular Imaging Using a High-frequency Ultrasound (30/45MHZ) System
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Entwicklungsbiologie
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JoVE Journal Entwicklungsbiologie
Fetal Mouse Cardiovascular Imaging Using a High-frequency Ultrasound (30/45MHZ) System

Fetal Mouse Cardiovascular Imaging Using a High-frequency Ultrasound (30/45MHZ) System

DOI:
10.3791/57210-v

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07:34 min

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May 05, 2018

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1Neonatal/Congenital Heart Laboratory, Cardiovascular Research Laboratory, David Geffen School of Medicine,University of California, Los Angeles, 2Children’s Discovery and Innovation Institute, Department of Pediatrics, David Geffen School of Medicine,University of California, Los Angeles

Kapitel

  • 00:05Titel
  • 00:45Preparing the High Frequency Ultrasound Imaging System
  • 01:36Preparing the Pregnant Mouse
  • 02:35Embryo Identification
  • 03:09Fetal Heart Visualization and Annotation
  • 05:05Evaluating Cardiopulmonary Flow Parameters
  • 05:44Evaluating the Feto-placental Axis
  • 06:30Results: Hemodynamic Parameters of Normoxic and Hypoxic Fetal Mice at Gestational Day 18.5
  • 06:56Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

High-frequency ultrasound imaging of the fetal mouse has improved imaging resolution and can provide precise non-invasive characterization of cardiac development and structural defects. The protocol outlined herein is designed to perform real-time fetal mice echocardiography in vivo.

Tags

Keywords: Fetal MouseCardiovascular ImagingHigh-frequency UltrasoundCardiac MorphologyFlow IndicesCongenital Heart Defects30/45MHz2D Dynamic ImagingAnesthesiaElectrocardiographyTemperature MonitoringFur RemovalFetal Orientation

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