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分离块茎原生质体流式细胞仪测量 Endoreduplication 的研究
JoVE Journal
Biologie
This content is Free Access.
JoVE Journal Biologie
Measuring Endoreduplication by Flow Cytometry of Isolated Tuber Protoplasts
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分离块茎原生质体流式细胞仪测量 Endoreduplication 的研究

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08:54 min

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March 09, 2018

DOI:

10.3791/57134-v

DOI:
10.3791/57134-v

•

08:54 min
March 09, 2018

•

2 Views
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1Department of Horticulture,Virginia Tech, 2Department of Biomedical Sciences and Pathobiology, Center for Molecular Medicine and Infectious Diseases, Virginia-Maryland Regional College of Veterinary Medicine,Virginia Tech

Summary

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本文描述的协议是一种测量马铃薯块茎内 endoreduplication 的方法 (马铃薯)。它包括质壁分离和原生质体提取步骤, 以减少下游流细胞分析中的噪声和碎片。

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Cite this Article

Laimbeer, F. P. E., Makris, M., Veilleux, R. E. Measuring Endoreduplication by Flow Cytometry of Isolated Tuber Protoplasts. J. Vis. Exp. (133), e57134, doi:10.3791/57134 (2018).

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