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使用磁纳米粒子转导病毒载体进行冷冻切片的原小鼠肠道器官的遗传工程
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07:37 min
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May 10, 2019
DOI:
1Department of Medicine, Division of Hematology,Johns Hopkins University School of Medicine, 2Department of Natural Sciences, School of Arts & Sciences,Lebanese American University, 3Department of Cell Biology,Johns Hopkins University School of Medicine, 4Department of Oncology,Johns Hopkins University School of Medicine, 5Department of Pathology,Johns Hopkins University School of Medicine, 6Institute for Cell Engineering,Johns Hopkins University School of Medicine
Summary
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我们描述了分步说明:1) 使用磁性纳米粒子对慢透或抗逆转录病毒转导进行高效工程肠道有机体,以及 2) 从工程有机物生成冷冻部分。这种方法提供了一个强大的工具,有效地改变基因表达在有机体的研究下游效应。
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Cite this Article
Xian, L., Chia, L., Georgess, D., Luo, L., Shuai, S., Ewald, A. J., Resar, L. M. Genetic Engineering of Primary Mouse Intestinal Organoids Using Magnetic Nanoparticle Transduction Viral Vectors for Frozen Sectioning. J. Vis. Exp. (147), e57040, doi:10.3791/57040 (2019).
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