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Etablering av en ekstracellulære surt pH kultur-systemet
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JoVE Journal Biologie
Establishment of an Extracellular Acidic pH Culture System

Etablering av en ekstracellulære surt pH kultur-systemet

DOI:
10.3791/56660-v

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09:41 min

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November 19, 2017

•

,
1Innovative Technology Laboratories,Kyowa Hakko Kirin Co., Ltd, 2Laboratory for Systems Biology and Medicine, RCAST,The University of Tokyo

Kapitel

  • 00:05Titel
  • 00:57Preparation of Acidic Culture Medium
  • 02:50Harvesting and Treating Cells
  • 04:31RNA Isolation
  • 06:30cDNA Synthesis and Analysis
  • 08:01Results: Transcriptional Upregulation of Acidic responsive Genes in Response to Lactic Acidosis and HCl-mediated Acidosis
  • 08:49Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

Sure svulst microenvironment spiller avgjørende roller i svulst progresjon. For å vurdere effekten av surt ekstracellulære pH på kreft celler i vitro, etablerte vi enkel surt kultur systemer.

Tags

Keywords: Extracellular Acidic PHTumor MicroenvironmentCancer ProgressionMalignancyAcidosisDMEMSodium BicarbonateL-glutaminePenicillin-streptomycinFetal Bovine SerumLactateHClPH MeasurementCell CulturePBSTrypsin

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