Journal
/
Biochemie
/
Isolation and Respiratory Measurements of Mitochondria from Arabidopsis thaliana
JoVE Journal
Biochemie
Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich.  Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
JoVE Journal Biochemie
Isolation and Respiratory Measurements of Mitochondria from Arabidopsis thaliana

Isolation and Respiratory Measurements of Mitochondria from Arabidopsis thaliana

DOI:
10.3791/56627-v

•

09:54 min

•

January 05, 2018

•

, , , , , ,
1ARC Centre of Excellence in Plant Energy Biology, Department of Animal, Plant and Soil Science, School of Life Science,La Trobe University, 2School of Biological Sciences,Flinders University, 3ARC Centre of Excellence in Plant Energy Biology,University of Western Australia

Kapitel

  • 00:05Titel
  • 00:50Preparation of Grinding Medium, Wash Buffer, and Gradient Solutions
  • 02:22Homogenization and Mitochondrial Isolation
  • 06:30Oxygen Consumption Measuurements
  • 08:42Results: Isolation of Purified Mitochondrial Protein and Oxygen Consumption Measurement of Purified Mitochondria Obtained from Arabidopsis thaliana Plants
  • 09:37Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

As mitochondria are only a small percentage of the plant cell, they need to be purified for a range of studies. Mitochondria can be isolated from a variety of plant organs by homogenization, followed by differential and density gradient centrifugation to obtain a highly purified mitochondrial fraction.

Tags

Mitochondria IsolationArabidopsis ThalianaRespirationMitochondrial CompositionBiogenesisMetabolismEnergy BiologyStress ToleranceGradient CentrifugationPVP GradientGrindingFiltration

Article

Embed

ZUR WIEDERGABELISTE HINZUFÜGEN

Usage Statistics

Verwandte Videos

Analyses of Mitochondrial Calcium Influx in Isolated Mitochondria and Cultured Cells

Rapid Isolation of the Mitoribosome from HEK Cells

Isolation of F1-ATPase from the Parasitic Protist Trypanosoma brucei

Preparation of Chloroplast Sub-compartments from Arabidopsis for the Analysis of Protein Localization by Immunoblotting or Proteomics

Isolation of Tissue Extracellular Vesicles from the Liver

Hybrid Clear/Blue Native Electrophoresis for the Separation and Analysis of Mitochondrial Respiratory Chain Supercomplexes

Mitochondria and Endoplasmic Reticulum Imaging by Correlative Light and Volume Electron Microscopy

Real-Time, Semi-Automated Fluorescent Measurement of the Airway Surface Liquid pH of Primary Human Airway Epithelial Cells

Interactions with and Membrane Permeabilization of Brain Mitochondria by Amyloid Fibrils

Generation and Assembly of Virus-Specific Nucleocapsids of the Respiratory Syncytial Virus

Read Article