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Focal Macropatch Recordings of Synaptic Currents from the Drosophila Larval Neuromuscular Junction
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JoVE Journal Neurowissenschaften
Focal Macropatch Recordings of Synaptic Currents from the Drosophila Larval Neuromuscular Junction

Focal Macropatch Recordings of Synaptic Currents from the Drosophila Larval Neuromuscular Junction

DOI:
10.3791/56493-v

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07:01 min

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September 25, 2017

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,
1Department of Neurology, School of Medicine,Wayne State University, 2Department of Anatomy and Cell Biology, School of Medicine,Wayne State University

Kapitel

  • 00:05Titel
  • 00:39Fabrication of Recording Electrodes
  • 01:53Additional Preparation
  • 03:17EJC Electrical Recording
  • 04:33EJC Electrical Stimulation and Seal Resistance
  • 05:34Results: Accurate Detection of Release Events
  • 06:29Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

Synaptic currents can be recorded focally from visualized synaptic boutons at the Drosophila third instar larvae neuromuscular junction. This technique enables monitoring the activity of a single synaptic bouton.

Tags

DrosophilaLarval Neuromuscular JunctionSynaptic CurrentsFocal Macropatch RecordingsSynaptic BoutonsSynaptic TransmissionSynaptic ProteinsMicroelectrodeHemolymph-like SolutionHL3 SolutionStimulation PipettesDissection PlatesWandering Third Instar LarvaeFiletingPinningNerve Cutting

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