Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich. Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
1School of Public Health of Southeast University,Laboratory of Environment and Biosafety Research Institute of Southeast University in Suzhou, 2Key Laboratory of Child Development and Learning Science (Ministry of Education), School of Biological Science & Medical Engineering,Southeast University, 3School of Public Health,Tianjin Medical University, 4British Columbia Academy,Nanjing Foreign Language School
Kapitel
- 00:04Titel
- 01:27Preparation of the Columns and Solutions Needed for the Extraction and Determination of Catecholamines
- 04:44Preparation of Real Urine Samples and Mobile Phase
- 06:25PFSPE Extraction and HPLC Analysis
- 09:58Results: Identification and Quantification of Extracted Catecholamines and their Metabolites in Urine
- 11:43Conclusion
Vi presenterer en praktisk solid-fase utvinning koblet til høytrykks flytende kromatografi (HPLC) med elektrokjemiske deteksjon (ECD) samtidige fastsettelse av tre monoamin nevrotransmittere, og to av deres metabolitter i spedbarn urin. Vi har også identifisere metabolitten MHPG som en potensiell biomarkør for tidlig diagnostikk av hjerneskade for spedbarn.
