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活细胞 Sod1 突变蛋白聚集物高含量定量测定方法的建立
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JoVE Journal Neurowissenschaften
Assay Development for High Content Quantification of Sod1 Mutant Protein Aggregate Formation in Living Cells
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活细胞 Sod1 突变蛋白聚集物高含量定量测定方法的建立

7,062 Views

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11:12 min

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October 04, 2017

DOI:

10.3791/56425-v

DOI:
10.3791/56425-v

•

11:12 min
October 04, 2017

•

3 Views
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1Automation & Logistics Management, Screening Sciences & Novel Assay Technologies,Institut Pasteur Korea, 2School of Pharmacy,Sungkyunkwan University, 3Technology Development Platform,Institut Pasteur Korea

Summary

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我们描述一种方法来量化错误蛋白质的聚集。我们的协议细节慢诱导稳定细胞线生成, 自动共焦成像, 和图像分析的蛋白质集料。作为一个说明性应用, 我们研究了小分子在促进 SOD1 聚集的时间和剂量依赖性的方式的影响。

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Lee, H., Radu, C., Han, J. W., Grailhe, R. Assay Development for High Content Quantification of Sod1 Mutant Protein Aggregate Formation in Living Cells. J. Vis. Exp. (128), e56425, doi:10.3791/56425 (2017).

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