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Imagens ao vivo da proteína GLUT4 tráfico de Mouse de neurônios hipotálamo primário usando microscopia de deconvolução
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Imagens ao vivo da proteína GLUT4 tráfico de Mouse de neurônios hipotálamo primário usando microscopia de deconvolução
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08:47 min
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December 07, 2017
DOI:
1The Ph.D. Program for Cancer Biology and Drug Discovery, College of Medical Science and Technology,Taipei Medical University, 2Integrated Laboratory, Center of Translational Medicine,Taipei Medical University, 3Core Facility,Taipei Medical University, 4The Ph.D. Program for Neural Regenerative Medicine, College of Medical Science and Technology,Taipei Medical University, 5Graduate Institute of Neural Regenerative Medicine, College of Medical Science and Technology,Taipei Medical University, 6TMU research center for Neurotrauma and Neuroregeneration, College of Medical Science and Technology,Taipei Medical University
Summary
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Este protocolo descreve uma técnica para observação de tempo real verde fluorescência proteína (GFP) com a tag 4 de transportador de glicose (GLUT4) proteína tráfico após estimulação da insulina e caracterização da função biológica de CCR5 na insulina – GLUT4 sinalização via com microscopia de deconvolução.
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Changou, C. A., Ajoy, R., Chou, S. Live Images of GLUT4 Protein Trafficking in Mouse Primary Hypothalamic Neurons Using Deconvolution Microscopy. J. Vis. Exp. (130), e56409, doi:10.3791/56409 (2017).
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