Journal
/
Neurowissenschaften
/
In Vitro Phagocytosis of Myelin Debris by Bone Marrow-Derived Macrophages
JoVE Journal
Neurowissenschaften
Author Produced
Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich.  Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
JoVE Journal Neurowissenschaften
In Vitro Phagocytosis of Myelin Debris by Bone Marrow-Derived Macrophages

In Vitro Phagocytosis of Myelin Debris by Bone Marrow-Derived Macrophages

DOI:
10.3791/56322-v

•

12:27 min

•

December 30, 2017

•

, , ,
1Department of Biomedical Sciences, College of Medicine,Florida State University

Kapitel

  • 00:00Titel
  • 00:09Introduction
  • 00:37Generation of Primary Bone Marrow-Derived Macrophages
  • 02:58Generation of Fluorescently Labeled Brain-Derived Myelin Debris
  • 08:51Representative Results
  • 11:01Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

We present methods to assess the phagocytic capacity of primary murine bone marrow-derived macrophages using fluorescently labeled myelin debris and intracellular lipid droplet staining.

Tags

In Vitro PhagocytosisMyelin DebrisBone Marrow-derived MacrophagesNeurotraumaMacrophage PhagocytosisMacrophage ResponsesCrude Myelin DebrisSucrose Gradient Ultracentrifugation

Article

Embed

ZUR WIEDERGABELISTE HINZUFÜGEN

Usage Statistics

Verwandte Videos

Transfection of Mouse Retinal Ganglion Cells by in vivo Electroporation

Inducing Plasticity of Astrocytic Receptors by Manipulation of Neuronal Firing Rates

Simultaneous ex vivo Functional Testing of Two Retinas by in vivo Electroretinogram System

A Novel In Vitro Live-imaging Assay of Astrocyte-mediated Phagocytosis Using pH Indicator-conjugated Synaptosomes

Ocular Kinematics Measured by In Vitro Stimulation of the Cranial Nerves in the Turtle

Identification of Dopamine D1-Alpha Receptor Within Rodent Nucleus Accumbens by an Innovative RNA In Situ Detection Technology

Modeling Charcot-Marie-Tooth Disease In Vitro by Transfecting Mouse Primary Motoneurons

Disruption of Frontal Lobe Neural Synchrony During Cognitive Control by Alcohol Intoxication

Real-time In Vitro Monitoring of Odorant Receptor Activation by an Odorant in the Vapor Phase

Rapid Isolation of Dorsal Root Ganglion Macrophages

Read Article