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Imaging neurale activiteit in de primaire somatosensorische Cortex met behulp van Thy1-GCaMP6s transgene muizen
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Imaging neurale activiteit in de primaire somatosensorische Cortex met behulp van Thy1-GCaMP6s transgene muizen
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January 07, 2019
DOI:
1Spinal Cord and Brain Injury Research Group, Stark Neurosciences Research Institute, Department of Neurological Surgery and Goodman and Campbell Brain and Spine, Department of Anatomy and Cell Biology,Indiana University School of Medicine, 2Department of Spinal Cord Injury and Repair, Trauma and Orthopedics Institute of Chinese PLA,General Hospital of Jinan Military Region, 3Department of Orthopedics, Shandong Cancer Hospital,Shandong University, 4Department of Neurobiology, Institute of Basic Medical Sciences,Academy Military Medical Sciences
Summary
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We beschrijven een experimentele procedure voor het meten van neuronale activiteit door dual optische ramen boven bilaterale primaire somatosensorische corticies (S1) in Thy1-GCaMP6s transgene muizen met behulp van 2-foton (2 P) microscopie in vivo.
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Lin, X., Zhao, T., Xiong, W., Wen, S., Jin, X., Xu, X. Imaging Neural Activity in the Primary Somatosensory Cortex Using Thy1-GCaMP6s Transgenic Mice. J. Vis. Exp. (143), e56297, doi:10.3791/56297 (2019).
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