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Regeneration of Arrayed Gold Microelectrodes Equipped for a Real-Time Cell Analyzer
1School of Public Health,Nanjing Medical University, 2School of Pharmacy,Nanjing Medical University, 3State Key Laboratory of Materials-Oriented Chemical Engineering, College of Chemistry and Chemical Engineering,Nanjing Tech University, 4Department of Medical Oncology, Jiangsu Cancer Hospital, Jiangsu Institute of Cancer Research,The Affiliated Cancer Hospital of Nanjing Medical University
Kapitel
- 00:04Titel
- 00:52Proliferation/Cytotoxicity Experiment and Data Acquisition
- 03:54Case 1: Regeneration of Electronic Plates after Cytotoxicity Evaluation of Anti-cancer Drug
- 05:53Case 2: Regeneration of Electronic Plates after Drug Delivery using Mesoporous Material
- 07:34Assessment of Regeneration Effect
- 10:22Results: Evaluationof Regenerated Electronic Plates
- 12:01Conclusion
This protocol describes a general strategy to regenerate commercial arrayed gold microelectrodes equipped for a label-free cell analyzer aimed at saving on the high running costs ofmicrochip-based assays. The regeneration process includes trypsin digestion, rinsing with ethanol and water, and a spinning step, which enables repeated usage of microchips.
