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斑马鱼幼虫动物模型的建立研究锥虫运动性体内
JoVE Journal
Entwicklungsbiologie
Author Produced
This content is Free Access.
JoVE Journal Entwicklungsbiologie
Establishment of Larval Zebrafish as an Animal Model to Investigate Trypanosoma cruzi Motility In Vivo
DOI:

13:21 min

September 30, 2017

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Kapitel

  • 00:00Titel
  • 00:13Introduction
  • 03:09Obtaining Embryos for Injection
  • 04:13Preparing Injection Material
  • 05:38Obtaining Labeled T. cruzi Parasites for Injection
  • 07:04Injecting Zebrafish Larvae
  • 08:10LSFM Mounting and Imaging of Injected Larvae
  • 08:57LSFM Imaging of Injected Larvae
  • 10:44Representative Results
  • 11:40Conclusion

Summary

Automatische Übersetzung

在本协议中, 荧光标记为T. 虫被注射到透明的斑马鱼幼虫中, 并使用光片荧光显微镜观察到体内的寄生虫运动。

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