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Journal
/
Entwicklungsbiologie
/
斑马鱼幼虫动物模型的建立研究
锥虫
运动性
体内
JoVE Journal
Entwicklungsbiologie
Author Produced
This content is Free Access.
JoVE Journal
Entwicklungsbiologie
Establishment of Larval Zebrafish as an Animal Model to Investigate
Trypanosoma cruzi
Motility
In Vivo
Please note that all translations are automatically generated.
Click here for the English version.
斑马鱼幼虫动物模型的建立研究
锥虫
运动性
体内
DOI:
10.3791/56238-v
•
13:21 min
•
September 30, 2017
•
Veronica Akle
,
Nathalie Agudelo-Dueñas*
1,2
,
Maria A. Molina-Rodriguez*
1
,
Laurel Brianne Kartchner
3,4,6
,
Annette Marie Ruth
3,5,6
,
John M. González
,
Manu Forero-Shelton
1
Laboratory of Neurosciences and Circadian Rhythms, School of Medicine
,
Universidad de los Andes
,
2
Biophysics Group, Department of Physics
,
Universidad de los Andes
,
3
Laboratory of Basic Medical Sciences, School of Medicine
,
Universidad de los Andes
,
4
Department of Microbiology and Immunology
,
University of North Carolina
,
5
Notre Dame Initiative for Global Development
,
University of Notre Dame
,
6
USAID Research and Innovation Fellowship program
Kapitel
00:00
Titel
00:13
Introduction
03:09
Obtaining Embryos for Injection
04:13
Preparing Injection Material
05:38
Obtaining Labeled
T. cruzi
Parasites for Injection
07:04
Injecting Zebrafish Larvae
08:10
LSFM Mounting and Imaging of Injected Larvae
08:57
LSFM Imaging of Injected Larvae
10:44
Representative Results
11:40
Conclusion
Summary
Automatische Übersetzung
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Automatische Übersetzung
在本协议中, 荧光标记为
T. 虫
被注射到透明的斑马鱼幼虫中, 并使用光片荧光显微镜观察到
体内的
寄生虫运动。
Tags
Trypanosoma Cruzi
Chagas Disease
Zebrafish Larvae
In Vivo Model
Parasite Motility
Light Sheet Fluorescence Microscopy
Transparent Casper Line
Host-pathogen Interactions
Article
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