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Entwicklungsbiologie

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成人果蝇脑内蘑菇体和感光神经元的解剖和荧光染色

JoVE Journal
Entwicklungsbiologie

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JoVE Journal Entwicklungsbiologie

Dissection and Immunofluorescent Staining of Mushroom Body and Photoreceptor Neurons in Adult Drosophila melanogaster Brains

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成人果蝇脑内蘑菇体和感光神经元的解剖和荧光染色

DOI:

10.3791/56174-v

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10:13 min

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November 06, 2017

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Seth M. Kelly², Alexandra Elchert, Michael Kahl³

¹Program in Neuroscience,The College of Wooster, ²Department of Biology,The College of Wooster, ³Program in Biochemistry, Cellular, and Molecular Biology,The College of Wooster

Kapitel

00:05Titel
01:13Adult Brain Dissection
03:39Brain Fixation and Immunofluorescent Staining
06:09Mounting Adult D. melanogaster Brains onto Microscope Slides and Imaging
07:47Results: Analyzing Axon Guidance Defects with the Mushroom Bodies
09:04Conclusion

Summary

Automatische Übersetzung

本协议描述了成人果蝇脑组织的解剖和免疫。具体地说, 该协议强调了使用果蝇蘑菇体和感光神经元作为神经元子集的例子, 可以准确地用来揭示神经发育的许多方面的一般原则。

Tags

Keywords: Drosophila Melanogaster Brain Dissection Immunofluorescence GFP Entwicklungsbiologie Neurowissenschaften Protein Localization Neuronal Connectivity Brain Development Morphological Defects Exoskeleton Anesthetized Flies PTN Proboscis Retina Optic Lobe Trachea

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