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Kapitel
- 00:05Titel
- 00:46Isolation of mRNA from Cells
- 03:245′ End Phosphorylation, DNase Treatment, and 3′ End Blocking
- 04:30Ligation of Reverse 3′ Adapters to 5′ End of RNA Fragments and Reverse Transcription
- 05:59Digestion with Uracil DNA Glycosylase Enzyme Mix and Ligation of 5′ Adapters to 5′ Ends of cDNA
- 07:44Pilot PCR, Amplification of Libraries, and Size Selection
- 09:21Computational Analysis of DNA Sequence Data
- 10:03Results: The A-seq2 Method Maps pre-mRNA 3′ End Processing Sites
- 10:52Conclusion
