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3' конца последовательности подготовки библиотека с A-seq2

JoVE Journal
Biologie

This content is Free Access.

JoVE Journal Biologie

3′ End Sequencing Library Preparation with A-seq2

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3' конца последовательности подготовки библиотека с A-seq2

10,076 Views

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12:01 min

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October 10, 2017

DOI:

10.3791/56129-v

DOI:

10.3791/56129-v

•

12:01 min

October 10, 2017

•

3 Views

Georges Martin, Ralf Schmidt, Andreas J. Gruber, Souvik Ghosh, Walter Keller, Mihaela Zavolan²

¹Computational and Systems Biology, Biozentrum,University of Basel, ²Swiss Institute of Bioinformatics, Biozentrum,University of Basel

Tags

3′ End Sequencing A-seq2 MRNA Processing Polyadenylation Transcript Isoforms Cell Types Poly(A) Stretches Adapter Dimers Cell Lysis Oligo D(T)25 Magnetic Beads Buffer A Buffer B Alkaline Hydrolysis RNA Isolation Polynucleotide Kinase

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