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Immunology and Infection

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JoVE Journal Immunology and Infection
Generation of Escape Variants of Neutralizing Influenza Virus Monoclonal Antibodies
 

Generation of Escape Variants of Neutralizing Influenza Virus Monoclonal Antibodies

Article DOI: 10.3791/56067-v • 07:55 min • August 29th, 2017
August 29th, 2017 •
1,2, 1,2, 1,2, 1,2, 3, 3, 1, 1
1Department of Microbiology, Icahn School of Medicine at Mount Sinai, 2Graduate School of Biomedical Sciences, Icahn School of Medicine at Mount Sinai, 3The Department of Medicine, Section of Rheumatology, The Knapp Center for Lupus and Immunology Research, The University of Chicago

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Summary
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We describe a method by which we identify critical residues required for the binding of human or murine monoclonal antibodies that target the viral hemagglutinin of influenza A viruses. The protocol can be adapted to other virus surface glycoproteins and their corresponding neutralizing antibodies.

Tags

Escape Variants Neutralizing Influenza Virus Monoclonal Antibodies Epitope Elucidation Immunology Virology Host-virus Interactions Therapeutics Diagnostic Tools Vaccines Tissue Culture Molecular Biology Techniques Characterization Of Antibodies Hemoagglutination Inhibition (HI) Neutralization Activities Antibody Dilutions Virus Stock Plaque-forming Units (PFU) Incubation
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