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Kronik Lenfositik Lösemi Hastalarında Metil-CpG-Bağlayıcı Alan Yakalamaya Dayalı Bir Yöntem Kullanarak Kapsamlı DNA Metilasyon Analizi
Kapitel
- 00:05Titel
- 00:49Genomic DNA Extraction and Sonication
- 02:06Bead Preparation Prior to Binding with Methyl-CpG-binding Domain (MBD)-biotin Protein
- 03:35Binding the MBD-biotin Protein to the Washed Beads
- 04:59Binding MBD-biotin Beads with Fragmented Genomic DNA
- 06:22Removing the Unbound DNA and Eluting the Methylated DNA from the Beads
- 08:10Ethanol Precipitation and the Enrichment of Methylated DNA
- 10:09Results: Identification of Differentially Methylated CpG Rich Regions in Chronic Lymphocytic Leukemia (CLL) Samples
- 11:26Conclusion
Tags
DNA MethylationMethyl-CpG-binding DomainCapture-based MethodChronic Lymphocytic LeukemiaNext-generation SequencingGlobal Methylation ProfilingDifferentially Methylated CpG-rich RegionsCLL-specific Signature GenesDisease PathogenesisPrognosisGenomic DNA ExtractionSonicationMagnetic Streptavidin BeadsMBD-biotin Protein
