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将斑马鱼小儿脑肿瘤移植入免疫能力的宿主,用于长期研究肿瘤细胞行为和药物反应
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Krebsforschung
Transplantation of Zebrafish Pediatric Brain Tumors into Immune-competent Hosts for Long-term Study of Tumor Cell Behavior and Drug Response
Please note that all translations are automatically generated.
Click here for the English version.
将斑马鱼小儿脑肿瘤移植入免疫能力的宿主,用于长期研究肿瘤细胞行为和药物反应
DOI:
10.3791/55712-v
•
09:43 min
•
May 17, 2017
•
Mattie J. Casey*
1
,
Katarzyna Modzelewska*
1
,
Daniela Anderson
,
James Goodman
,
Elena F. Boer
,
Laura Jimenez
,
Douglas Grossman
2
,
Rodney A. Stewart
1
Department of Oncological Sciences and Huntsman Cancer Institute
,
University of Utah School of Medicine
,
2
Department of Dermatology
,
University of Utah Health Sciences Center, Salt Lake City
Kapitel
00:05
Titel
01:07
Preparing Equipment for Microinjection
02:46
Making a Tumor Cell Suspension
04:22
Injecting Tumor Suspension into the Fourth Ventricle of a 2-dpf Embryo
07:25
Results: Brain Tumor Transplantation in Zebrafish
08:57
Conclusion
Summary
Automatische Übersetzung
English (Original)
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Automatische Übersetzung
癌细胞的移植是识别癌症机制和治疗反应的重要工具。目前的技术取决于免疫力不足的动物。在这里,我们描述了一种将斑马鱼肿瘤细胞移植到免疫感受态胚胎中的方法,用于长期分析肿瘤细胞行为和
体内
药物反应。
Tags
Zebrafish
Pediatric Brain Tumors
Immune-competent Hosts
Tumor Cell Behavior
Drug Response
Invasion
Dissemination
Brain Cancer Biology
Therapy
Tumor Burden
Agarose
Methylene Blue
Injection Plate
Needle Puller
Capillaries
Razor Blade
Tumor Mass
Cell Suspension
Centrifugation
Article
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