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Analisi istologiche di lesioni al fegato acuto alcolico in Zebrafish
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Entwicklungsbiologie
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JoVE Journal Entwicklungsbiologie
Histological Analyses of Acute Alcoholic Liver Injury in Zebrafish
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Analisi istologiche di lesioni al fegato acuto alcolico in Zebrafish

13,831 Views

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10:45 min

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May 25, 2017

DOI:

10.3791/55630-v

DOI:
10.3791/55630-v

•

10:45 min
May 25, 2017

•

59 Views
,
1Division of Gastroenterology, Hepatology and Nutrition,Cincinnati Children’s Hospital Medical Center, 2Division of Developmental Biology,Cincinnati Children’s Hospital Medical Center

Summary

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Questo protocollo descrive le analisi istologiche dei fegati delle larve di zebrafish che sono state trattate con 2% di etanolo per 24 ore. Tale trattamento di etanolo acuto provoca steatosi epatica e gonfiore della vasculatura epatica.

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ZebrafishHistologyAcute Alcoholic Liver InjuryPatho-histologyEthanolAlcohol Liver Disease2% EthanolDietrich’s FixativePBS3% AgaroseSagittal Sections

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Cite this Article

Ellis, J. L., Yin, C. Histological Analyses of Acute Alcoholic Liver Injury in Zebrafish. J. Vis. Exp. (123), e55630, doi:10.3791/55630 (2017).

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