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A 5-mC Dot Blot Assay Quantifying the DNA Methylation Level of Chondrocyte Dedifferentiation In Vitro
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A 5-mC Dot Blot Assay Quantifying the DNA Methylation Level of Chondrocyte Dedifferentiation In Vitro
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10:07 min
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May 17, 2017
DOI:
1Guangzhou Medical University, 2Shenzhen Key Laboratory of Tissue Engineering, Shenzhen Laboratory of Digital Orthopeadic Engineering, Department of Orthopedics,Shenzhen Second People’s Hospital (The First Hospital Affiliated to Shenzhen University), 3Department of Chemistry,The Chinese University of Hong Kong, 4School of Biomedical Engineering,Shanghai Jiao Tong University, 5Renji Hospital Clinical Stem Cell Research Center,Shanghai Jiao Tong University School of Medicine, 6Shantou University Medical College
Summary
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We present a method to quantify DNA methylation based on the 5-methylcytosine (5-mC) dot blot. We determined the 5-mC levels during chondrocyte dedifferentiation. This simple technique could be used to quickly determine the chondrocyte phenotype in ACI treatment.
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Cite this Article
Jia, Z., Liang, Y., Ma, B., Xu, X., Xiong, J., Duan, L., Wang, D. A 5-mC Dot Blot Assay Quantifying the DNA Methylation Level of Chondrocyte Dedifferentiation In Vitro. J. Vis. Exp. (123), e55565, doi:10.3791/55565 (2017).
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