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Dorsalrotganglion Injeksjon og rygg Root knusningsskade som en modell for Sensory Axon Regeneration
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JoVE Journal Neurowissenschaften
Dorsal Root Ganglion Injection and Dorsal Root Crush Injury as a Model for Sensory Axon Regeneration

Dorsalrotganglion Injeksjon og rygg Root knusningsskade som en modell for Sensory Axon Regeneration

DOI:
10.3791/55535-v

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09:48 min

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May 03, 2017

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, ,
1John van Geest Center for Brain Repair,University of Cambridge, 2School of Medicine,University of St. Andrews, 3Department of Biological Sciences,University of Southampton

Kapitel

  • 00:05Titel
  • 00:48Preoperative Procedures
  • 01:45DRG Injection and Dorsal Root Crush Injury
  • 05:08Postoperative Procedures
  • 06:34Anterograde CTB Injection for Axonal Tracing
  • 07:25Results: Sensory Axon Regeneration Visualized with GFP and CTB after Dorsal Root Crush Injury
  • 08:59Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

This protocol presents the use of a dorsal root ganglion (DRG) injection with a viral vector and a concurrent dorsal root crush injury in an adult rat as a model to study sensory axon regeneration. This model is suitable for investigating the use of gene therapy to promote sensory axon regeneration.

Tags

Dorsal Root GanglionDorsal Root Crush InjurySensory Axon RegenerationSpinal CordAxon RegenerationCNS Axon RegenerationSurgical ApproachNeuronal Cell BodyTherapeutic InterventionAnimal WelfareSurgical ProcedureAnesthesiaAnalgesiaAntibioticStereotaxic FrameMuscle LayersSpinous ProcessesDissection

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