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Open source Single-partikel Analyse for Super-resolution Microscopy med VirusMapper
JoVE Journal
Biologie
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JoVE Journal Biologie
Open-source Single-particle Analysis for Super-resolution Microscopy with VirusMapper

Open source Single-partikel Analyse for Super-resolution Microscopy med VirusMapper

DOI:
10.3791/55471-v

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07:38 min

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April 09, 2017

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, ,
1MRC Laboratory for Molecular Cell Biology,University College London, 2Centre for Mathematics and Physics in Life Sciences and Experimental Biology (CoMPLEX),University College London, 3Department of Cell and Developmental Biology,University College London

Kapitel

  • 00:05Titel
  • 00:50Image Setup and Particle Extraction
  • 02:53Seed Generation and Selection
  • 04:32Model Generation from Seeds
  • 06:11Results: Modeling of Vaccinia with VirusMapper
  • 06:49Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

Dette håndskrift bruger Fiji-baserede open source software-pakke VirusMapper at anvende enkelt-partikel analyse til super-opløsning mikroskopi billeder for at generere præcise modeller af nanoskala struktur.

Tags

Single-particle AnalysisSuper-resolution MicroscopyVirusMapperVirus StructureMacromolecular ComplexesFluorescence MicroscopyImage ProcessingParticle ExtractionROI SelectionSeed Generation

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