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隔离并通过流式细胞仪分析胰腺间充质细胞
JoVE Journal
Entwicklungsbiologie
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JoVE Journal Entwicklungsbiologie
Isolating and Analyzing Cells of the Pancreas Mesenchyme by Flow Cytometry
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隔离并通过流式细胞仪分析胰腺间充质细胞

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05:38 min

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January 28, 2017

DOI:

10.3791/55344-v

DOI:
10.3791/55344-v

•

05:38 min
January 28, 2017

•

7 Views
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1Cell and Developmental Biology,Sackler Faculty of Medicine, Tel Aviv University

Summary

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这里，我们描述了一种用于细胞在胰腺微环境从胚胎，新生儿和成年小鼠组织的隔离，着眼于间充质细胞的分离。此方法允许细胞基因表达和蛋白质分泌的分析以阐明调节胰腺发育，功能，和肿瘤发生的外在信号。

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Cite this Article

Epshtein, A., Sakhneny, L., Landsman, L. Isolating and Analyzing Cells of the Pancreas Mesenchyme by Flow Cytometry. J. Vis. Exp. (119), e55344, doi:10.3791/55344 (2017).

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