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Kapitel
- 00:00Titel
- 00:41Protocol
- 01:06Isolation: Soil Dilution Method
- 02:29Isolation from Insect Cadavers
- 02:55Isolation: Bait Method
- 03:44Identification of Entomopathogenic Fungi
- 04:11Representative Results: Entomopathogenic Fungi
- 04:34Insect Bioassay of Metarhizium Isolates
- 06:23Representative Results: Metarhizium Isolates
- 06:59Production of Spores: Development of Mycelial Inoculum
- 07:33Production of Spores: Solid State Fermentation for Production of Spores
- 09:22Production of Spores: Precentatge Germination Studies
- 09:55Production of Spores: Spore Sedimentation Rate
- 10:32Representative Results: Production of Spores
- 10:41Field Performance of Metarhizium Isolate M34412: Aqueous and Oil Based Spore Formulations
- 11:59Representative Results: Effect of Metarhizium anisopliae on Non-target Anthropods
- 12:29Quality Control Parameteres
- 13:37Discussion and Conclusion
Here, we report the different stages involved in the knowledge-based development of an effective mycoinsecticide, including the isolation, identification, screening, and selection of the "best-fit" entomopathogenic fungus, Metarhizium anisopliae, for the control of insect pests in agriculture.
