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Immunology and Infection

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JoVE Journal Immunology and Infection
Dextran Enhances the Lentiviral Transduction Efficiency of Murine and Human Primary NK Cells
 

Dextran Enhances the Lentiviral Transduction Efficiency of Murine and Human Primary NK Cells

Article DOI: 10.3791/55063-v • 08:42 min • January 15th, 2018
January 15th, 2018 •
1, 2, 3, 4, 5, 6, 6, 1,5,6,7
1Laboratory of Molecular Immunology and Immunotherapy, Blood Research Institute, The Blood Center of Wisconsin, 2Vector Core Lab, Blood Research Institute, The Blood Center of Wisconsin, 3Laboratory of Lymphocyte Biology, Blood Research Institute, The Blood Center of Wisconsin, 4Laboratory of Stem Cell Transcriptional Regulation, Blood Research Institute, The Blood Center of Wisconsin, 5Department of Microbiology and Immunology, The Medical College of Wisconsin, 6Department of Pediatrics, The Medical College of Wisconsin, 7Department of Medicine, The Medical College of Wisconsin

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Summary
Automatic Translation

The goal of this study was to formulate technologies that allow for successful gene transduction in primary natural killer (NK) cells. The dextran-mediated lentiviral transduction of human or mouse primary NK cells results in higher gene expression efficiencies. This method of gene transduction will vastly improve NK cell genetic manipulation.

Tags

Keywords: Lentiviral Transduction Primary NK Cells Immunotherapy Gene Transduction Murine NK Cells Human NK Cells Cell Purification Flow Cytometry Viral Transduction Dextran Polybrene Protamine Sulfate
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